Septin 6 Polyklonaler Antikörper

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• KD/KO Validated

Septin 6 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12805-1-PBS

Synonyme

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  WB analysis of Jurkat using 12805-1-AP (same clone as 12805-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12805-1-AP (same clone as 12805-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 12805-1-AP (same clone as 12805-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 12805-1-AP (same clone as 12805-1-PBS)

  IP result of anti-Septin 6 (IP:12805-1-AP, 4ug; Detection:12805-1-AP 1:600) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 12805-1-AP (same clone as 12805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (Septin 6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12805-1-AP (same clone as 12805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (SEPT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12805-1-PBS bindet in WB, IHC, IP, Indirect ELISA Septin 6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 6 fusion protein Ag3521
• Vollständiger Name: septin 6
• Berechnetes Molekulargewicht: 434 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036240
• Gene symbol: SEPT6
• Gene ID (NCBI): 23157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.