Serotonin transporter Polyklonaler Antikörper

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• KD/KO Validated

Serotonin transporter Polyklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19559-1-PBS

Synonyme

SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT

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  WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19559-1-PBS bindet in WB, IF-P, Indirect ELISA Serotonin transporter und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa and 140 kDa
• GenBank-Zugangsnummer: NM_001045
• Gene symbol: Serotonin transporter
• Gene ID (NCBI): 6532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 140 kDa bands in SDS-PAGE. The 140 kDa band corresponds to the dimer of high mannose-type SERT (PMID: 19323988).