Serotonin transporter Rekombinanter Antikörper

Serotonin transporter Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242252B9

Kat-Nr. : 84844-3-PBS

Synonyme

SLC6A4, 242252B9, 5 HTT, 5 HTTLPR, 5HTT

---

• 

  WB analysis using 84844-3-RR (same clone as 84844-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84844-3-RR (Serotonin transporter antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84844-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84844-3-RR (same clone as 84844-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84844-3-RR (Serotonin transporter antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 84844-3-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84844-3-PBS bindet in WB, IHC, Indirect ELISA Serotonin transporter und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_001045
• Gene symbol: Serotonin transporter
• Gene ID (NCBI): 6532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, partly by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).