Serpin C1/Antithrombin III Monoklonaler Antikörper

Serpin C1/Antithrombin III Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B2E5

Kat-Nr. : 66052-1-PBS

Synonyme

Antithrombin III, Serpin C1, SERPINC1, 2B2E5, Antithrombin-III

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  WB analysis of human plasma using 66052-1-Ig (same clone as 66052-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Serpin C1/Antithrombin III antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66052-1-Ig (same clone as 66052-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66052-1-Ig (same clone as 66052-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66052-1-Ig (same clone as 66052-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66052-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Serpin C1/Antithrombin III und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Serpin C1/Antithrombin III fusion protein Ag9880
• Vollständiger Name: serpin peptidase inhibitor, clade C (antithrombin), member 1
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC022309
• Gene symbol: Serpin C1/Antithrombin III
• Gene ID (NCBI): 462
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.