Serpin E1/PAI-1 Rekombinanter Antikörper

Serpin E1/PAI-1 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241130G6

Kat-Nr. : 83980-3-PBS

Synonyme

PAI-1, Serpine1, 241130G6, PAI 1, PAI1

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  WB analysis of mouse placenta using 83980-3-RR (same clone as 83980-3-PBS)

  mouse placenta tissue was subjected to SDS PAGE followed by western blot with 83980-3-RR (Serpin E1/PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83980-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00875-4

  Sandwich ELISA standard curve of MP00875-4, Mouse Serpin E1/PAI-1 Recombinant Matched Antibody Pair - PBS only. 83980-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1100. 83980-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP00875-4

  L-929 mouse fibroblast cells (1 x 10^6 cells/mL) were cultured for 3 days in MEM supplemented with 10% equine serum and stimulated with 10 μg/mL PHA. The cell culture supernatants was assayed for mouse Serpin E1/PAI-1 and measured 41,666.9 pg/mL.

• 

  Sample test of MP00875-4

  Mouse heart tissue lyaste was measured. The mouse Serpin E1/PAI-1 concentration of detected samples was determined to be 1.46 ng/mL (based on a 4.6 mg/mL extract load).

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  Sample test of MP00875-4

  Serum of six mice was measured. The mouse Serpin E1/PAI-1 concentration of detected samples was determined to be 16,187.9 pg/mL with a range of 6,614.7 - 24,758.6 pg/mL.

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  Affinity and Kinetic Characterization of 83980-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83980-3-RR against Mouse Serpin E1/PAI-1 were performed. The affinity constant is 0.293 nM.

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Geprüfte Anwendungen

Produktinformation

83980-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Serpin E1/PAI-1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Serpin E1/PAI-1 fusion protein Eg1100
• Vollständiger Name: serine (or cysteine) peptidase inhibitor, clade E, member 1
• Berechnetes Molekulargewicht: 45kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• Gene symbol: Serpin E1/PAI-1
• Gene ID (NCBI): 18787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.