Serpin F1/PEDF Rekombinanter Antikörper

Serpin F1/PEDF Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242127F9

Kat-Nr. : 84685-3-RR

Synonyme

PEDF, PIG35, 242127F9, Cell proliferation-inducing gene 35 protein, EPC 1

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  WB analysis of HepG2 using 84685-3-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 84685-3-RR

  C2C12 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 84685-3-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Serpin F1/PEDF Recombinant antibody (84685-3-RR, Clone:242127F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84685-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84685-3-RR against Human Serpin F1/PEDF were performed. The affinity constant is 1.08 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C2C12-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84685-3-RR bindet in WB, FC (Intra), ELISA Serpin F1/PEDF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Berechnetes Molekulargewicht: 46kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_002615.7
• Gene symbol: PEDF
• Gene ID (NCBI): 5176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.