Serpina3k Polyklonaler Antikörper

Serpina3k Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 55480-1-AP

Synonyme

1300001I07Rik, contrapsin, D12Rp54, MMCM2, MMSpi2, RP54, Serpina3k, Spi 2, Spi2

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  WB analysis of mouse heart using 55480-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55480-1-AP (Serpina3k antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 55480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55480-1-AP (Serpina3k antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 55480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55480-1-AP (Serpina3k antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

55480-1-AP bindet in WB, IHC, IF, ELISA Serpina3k und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: serine (or cysteine) peptidase inhibitor, clade A, member 3K
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_011458
• Gene symbol: Serpina3k
• Gene ID (NCBI): 20714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Biol Toxicol; Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis.; Authors - Yaochen Cao
mouse	WB	Int J Mol Sci; Sirtuin 7 Deficiency Reduces Inflammation and Tubular Damage Induced by an Episode of Acute Kidney Injury.; Authors - Andrea Sánchez-Navarro
rat	WB	FASEB J; Transient response of serpinA3 during cellular stress.; Authors - Andrea Sánchez-Navarro
human	WB,IHC	Am J Physiol Renal Physiol; Urinary serpin-A3 is an early predictor of clinical response to therapy in patients with proliferative lupus nephritis; Authors - Miguel Ángel Martínez-Rojas
	WB,IHC	Sci Rep; SerpinA3 in the Early Recognition of Acute Kidney Injury to Chronic Kidney Disease (CKD) transition in the rat and its Potentiality in the Recognition of Patients with CKD.; Authors - Andrea Sánchez-Navarro
mouse	WB	Front Neurol; Neuroprotective Effects of Serpina3k in Traumatic Brain Injury.; Authors - Yao Jing