Serum amyloid P component Rekombinanter Antikörper

Serum amyloid P component Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241403F5

Kat-Nr. : 84402-1-PBS

Synonyme

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

---

• 

  WB analysis of human plasma using 84402-1-RR (same clone as 84402-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 84402-1-RR (Serum amyloid P component antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84402-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01275-1

  Sandwich ELISA standard curve of MP01275-1, Human Serum amyloid P component Recombinant Matched Antibody Pair - PBS only. 84402-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0528. 84402-1-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

• 

  Sample test of MP01275-1

  Serum of eight individual healthy human donors was measured. The human Serum amyloid P component concentration of detected samples was determined to be 32.91 µg/mL with a range of 16.86 - 48.68 µg/mL.

• 

  Affinity and Kinetic Characterization of 84402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84402-1-RR against Human Serum amyloid P component were performed. The affinity constant is 1.49 nM.

---

Geprüfte Anwendungen

Produktinformation

84402-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test Serum amyloid P component und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: amyloid P component, serum
• Berechnetes Molekulargewicht: 223 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27-29 kDa
• GenBank-Zugangsnummer: BC007058
• Gene symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.