Sestrin 2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Sestrin 2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A4

Kat-Nr. : 66297-1-PBS

Synonyme

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

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  WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

  WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66297-1-Ig (same clone as 66297-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66297-1-Ig (same clone as 66297-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66297-1-Ig (same clone as 66297-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66297-1-Ig (same clone as 66297-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66297-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Sestrin 2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Sestrin 2 fusion protein Ag20638
• Vollständiger Name: sestrin 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC013304
• Gene symbol: SESN2/Sestrin 2
• Gene ID (NCBI): 83667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).