Sestrin 2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Sestrin 2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

CloneNo.

1A1A4

Rezensionen (1)

Kat-Nr. : 66297-1-Ig

Synonyme

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293T using 66297-1-Ig

  WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells.

• 

  WB analysis of HeLa using 66297-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66297-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66297-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66297-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66297-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66297-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66297-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66297-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

66297-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA Sestrin 2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Sestrin 2 fusion protein Ag20638
• Vollständiger Name: sestrin 2
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC013304
• Gene symbol: SESN2
• Gene ID (NCBI): 83667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Publikationen

Species	Application	Title
human,mouse	WB,IF	Cell Biol Toxicol; Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.; Authors - Yiming Jiang ; KD Validated
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
pig	WB, IP	J Nutr; Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.; Authors - Agus Suryawan
human,mouse	WB,IHC,IF,IP	Cell Tissue Res; Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy.; Authors - Yawei Bian
pig	WB	Am J Physiol Endocrinol Metab; Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs.; Authors - Marko Rudar
mouse	WB	Arch Pharm Res; Sestrin2 induction contributes to anti-inflammatory responses and cell survival by globular adiponectin in macrophages; Authors - Sumin Lee ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yahir Alberto (Verified Customer) (11-12-2021)

This antibody works nicely for western blotting, although with several unspecific bands for a monoclonal antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T cells

Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:66297-1-Ig)