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Sestrin 2 Rekombinanter Antikörper

Sestrin 2 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240034H3

Kat-Nr. : 83202-3-PBS

Synonyme

SESN2, Sestrin2, 240034H3, EC:1.11.1.-, Hi95

Filter:
  • Western Blot (WB) analysis of various lysates using Sestrin 2 Recombinant antibody (83202-3-RR)

    WB analysis using 83202-3-RR (same clone as 83202-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Sestrin 2 Recombinant antibody (83202-3-RR)

    FC experiment of HEK-293 using 83202-3-RR (same clone as 83202-3-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00278-1

    Cytometric bead array standard curve of MP00278-1

    Cytometric bead array standard curve of MP00278-1, Sestrin 2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83202-2-PBS. Detection antibody: 83202-3-PBS. Standard: Ag1247. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83202-3-RR

    Affinity and Kinetic Characterization of 83202-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83202-3-RR against Human Sestrin 2 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

83202-3-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA Sestrin 2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Sestrin 2 fusion protein Ag1247
Vollständiger Name sestrin 2
Berechnetes Molekulargewicht 54 kDa
GenBank-ZugangsnummerBC013304
Gene symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.