Shootin 1 Rekombinanter Antikörper

Shootin 1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251435E5

Kat-Nr. : 86541-2-RR

Synonyme

SHTN1, KIAA1598, Shootin-1

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  WB analysis using 86541-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86541-2-RR (KIAA1598 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86541-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86541-2-RR (KIAA1598 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86541-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86541-2-RR against Human Shootin 1 were performed. The affinity constant is 0.965 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SK-N-SH cells, HeLa-Zellen, Neuro-2a-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86541-2-RR bindet in WB, ELISA Shootin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Shootin 1 fusion protein Ag18294
• Vollständiger Name: KIAA1598
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 60-80 kDa
• GenBank-Zugangsnummer: BC022348
• Gene symbol: Shootin 1
• Gene ID (NCBI): 57698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shootin 1, also known as SHTN1, KIAA1598, contains 3 coiled-coil domains and a proline-rich region. Shootin 1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. Shootin 1 plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. SHTN1 produces 2 splice variants, a short variant, SHTN1S, that lacks exons 15 and 16, and a long variant, SHTN1L, that includes exons 1 through 17(PMID: 17030985, 30733148).