Siglec-6/CD327 Rekombinanter Antikörper

Siglec-6/CD327 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

243088G2

Kat-Nr. : 98416-1-PBS

Synonyme

SIGLEC6, CD327, CD33 antigen like 1, CD33 antigen-like 1, CD33L

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  FC experiment of human PBMCs using 98416-1-RR (same clone as 98416-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98416-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98416-1-RR (same clone as 98416-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98416-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98416-1-PBS bindet in FC Siglec-6/CD327 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Siglec-6/CD327 fusion protein Eg2070
• Vollständiger Name: sialic acid binding Ig-like lectin 6
• Berechnetes Molekulargewicht: 426 aa, 47 kDa
• GenBank-Zugangsnummer: BC035359
• Gene symbol: SIGLEC6
• Gene ID (NCBI): 946
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Siglec-6, also known as CD327, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).