Siglec-6/CD327 Rekombinanter Antikörper

Siglec-6/CD327 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

243088G2

Kat-Nr. : 98416-1-RR

Synonyme

SIGLEC6, CD327, CD33 antigen like 1, CD33 antigen-like 1, CD33L

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  FC experiment of human PBMCs using 98416-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98416-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98416-1-RR bindet in FC Siglec-6/CD327 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Siglec-6/CD327 fusion protein Eg2070
• Vollständiger Name: sialic acid binding Ig-like lectin 6
• Berechnetes Molekulargewicht: 426 aa, 47 kDa
• GenBank-Zugangsnummer: BC035359
• Gene symbol: SIGLEC6
• Gene ID (NCBI): 946
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Siglec-6, also known as CD327, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).