Siglec-7/CD328 Rekombinanter Antikörper

Siglec-7/CD328 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241525G3

Kat-Nr. : 84253-4-RR

Synonyme

SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1

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  WB analysis of U-937 using 84253-4-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 84253-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84253-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84253-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84253-4-RR against Human Siglec-7/CD328 were performed. The affinity constant is 53.4 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84253-4-RR bindet in WB, IHC, ELISA Siglec-7/CD328 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: sialic acid binding Ig-like lectin 7
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_014385.4
• Gene symbol: Siglec-7
• Gene ID (NCBI): 27036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).