CD169 Rekombinanter Antikörper

CD169 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251348G9

Kat-Nr. : 86605-1-PBS

Synonyme

Sa, SER, Sheep erythrocyte receptor, Sialic acid-binding Ig-like lectin 1, Sialoadhesin

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  WB analysis using 86605-1-RR (same clone as 86605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86605-1-RR (Siglec1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86605-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02539-1

  The mean Sialoadhesin/CD169 concentration was determined to be 519.6 ng/mL in mouse spleen tissue extract based on a 8.5 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02539-1

  Sandwich ELISA standard curve of MP02539-1, Mouse Sialoadhesin/CD169 Recombinant Matched Antibody Pair - PBS only. 86605-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5002. 86605-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Cytometric bead array sample test of MP02539-1

  The mean Sialoadhesin/CD169 concentration was determined to be 351.7 ng/mL in Mouse spleen tissue extract based on a 6.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP02539-1

  Cytometric bead array standard curve of MP02539-1, MOUSE Sialoadhesin/CD169 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86605-1-PBS. Detection antibody: 86605-2-PBS. Standard: Eg5002. Range: 0.625-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86605-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA CD169 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD169 fusion protein Eg5002
• Vollständiger Name: sialic acid binding Ig-like lectin 1, sialoadhesin
• Berechnetes Molekulargewicht: 183 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: NP_035556
• Gene symbol: Siglec1
• Gene ID (NCBI): 20612
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 3783087; 11133773). CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail (PMID: 11133773). It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs (PMID: 33408860). CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.