Siglec-E Rekombinanter Antikörper

Siglec-E Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242248H3

Kat-Nr. : 84790-2-PBS

Synonyme

MIS, mSiglec-E, Myeloid inhibitory siglec, Sialic acid-binding Ig-like lectin 12, Sialic acid-binding Ig-like lectin 5

---

• 

  WB analysis of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01535-1

  Cytometric bead array standard curve of MP01535-1, MOUSE Siglec-12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84790-2-PBS. Detection antibody: 84790-1-PBS. Standard: Eg2733. Range: 3.125-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

84790-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA Siglec-E und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Siglec-E fusion protein Eg2733
• Vollständiger Name: sialic acid binding Ig-like lectin E
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 70-90 kDa
• GenBank-Zugangsnummer: NM_031181.2
• Gene symbol: Siglec-E
• Gene ID (NCBI): 83382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700).