Somatostatin (1-116aa) Polyklonaler Antikörper

Somatostatin (1-116aa) Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24496-1-AP

Synonyme

SMST, somatostatin, Somatostatin (1-116aa), SST, somatostatin

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  IHC staining of mouse pancreas using 24496-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24496-1-AP (Somatostatin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 24496-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24496-1-AP (Somatostatin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 24496-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin antibody (24496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red).

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  IF Staining of mouse pancreas using 24496-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (1-116aa) antibody (24496-1-AP) at dilution of 1:2000 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L), CoraLite®647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

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  IF Staining of mouse pancreas using 24496-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (1-116aa) antibody (24496-1-AP) at dilution of 1:2000 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L), CoraLite®647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24496-1-AP bindet in IHC, IF-P, ELISA Somatostatin (1-116aa) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Somatostatin (1-116aa) fusion protein Ag21294
• Vollständiger Name: somatostatin
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC032625
• Gene symbol: Somatostatin
• Gene ID (NCBI): 6750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

Publikationen

Species	Application	Title
		iScience; Mosaic ablation of pancreatic β cells induces de-differentiation and repetitive proliferation of residual β cells in adult mice; Authors - Ryo Hatano