Sortilin Polyklonaler Antikörper

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• KD/KO Validated

Sortilin Polyklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-12369

Synonyme

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

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  IF Staining of mouse brain using CL647-12369

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

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  IF Staining of mouse brain using CL647-12369

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

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  FC experiment of Neuro-2a using CL647-12369

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Sortilin (CL647-12369) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-12369 bindet in IF-P, FC (Intra) Sortilin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sortilin fusion protein Ag2997
• Vollständiger Name: sortilin 1
• Berechnetes Molekulargewicht: 831 aa, 92 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC023542
• Gene symbol: Sortilin
• Gene ID (NCBI): 6272
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.