Spag8 Polyklonaler Antikörper

Spag8 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30287-1-PBS

Synonyme

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  WB analysis using 30287-1-AP (same clone as 30287-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30287-1-AP (same clone as 30287-1-PBS)

  rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 30287-1-AP (same clone as 30287-1-PBS)

  IP result of anti-Spag8 (IP:30287-1-AP, 4ug; Detection:30287-1-AP 1:3000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 30287-1-AP (same clone as 30287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 30287-1-AP (same clone as 30287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30287-1-PBS bindet in WB, IHC, IP, Indirect ELISA Spag8 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Spag8 fusion protein Ag31603
• Vollständiger Name: sperm associated antigen 8
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC150868
• Gene symbol: Spag8
• Gene ID (NCBI): 433700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.