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Spartan Polyklonaler Antikörper

Spartan Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24850-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of human placenta tissue using Spartan Polyclonal antibody (24850-1-AP)

    WB analysis of human placenta using 24850-1-AP (same clone as 24850-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 24850-1-AP (Spartan antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24850-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Spartan Polyclonal antibody (24850-1-AP)

    IP experiment of HEK-293 using 24850-1-AP (same clone as 24850-1-PBS)

    IP result of anti-Spartan (IP:24850-1-AP, 4ug; Detection:24850-1-AP 1:500) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 24850-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24850-1-PBS bindet in WB, IP, Indirect ELISA Spartan und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Spartan fusion protein Ag20586
Vollständiger Name DNA-dependent metalloprotease SPRTN
Berechnetes Molekulargewicht 489 aa, 55 kDa
Beobachtetes Molekulargewicht55 kDa, 65 kDa
GenBank-ZugangsnummerBC015740
Gene symbol Spartan
Gene ID (NCBI) 83932
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spartan (Protein with SprT-like domain at the N terminus), also known as DVC1 or C1orf124, is a replication-coupled DNA-dependent metalloprotease that cleaves proteins cross-linked to DNA to promote DPC repair (PMID: 38600235). Spartan protease activity is stimulated by DNA binding, while posttranslational modification of Spartan governs both its protease activity and recruitment to the DPC on chromatin (PMID: 27871366). The regulation of Spartan includes a ubiquitin switch (where deubiquitination activates its proteolytic activity), and acetylation and phosphorylation switches (which facilitate DNA binding). The calculated molecular weight of Spartan is 55 kDa, while the 65-kDa band could be due to posttranslational modification (PMID: 33567341, 31316063).