Spartin/SPG20 Rekombinanter Antikörper

Spartin/SPG20 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250440A11

Kat-Nr. : 85938-2-RR

Synonyme

SPG20, KIAA0610, SPART, Spartin, SPG 20

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  WB analysis using 85938-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85938-2-RR (Spartin/SPG20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85938-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85938-2-RR against Human Spartin/SPG20 were performed. The affinity constant is 1.66 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HL-60-Zellen, MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85938-2-RR bindet in WB, ELISA Spartin/SPG20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Spartin/SPG20 fusion protein Ag4815
• Vollständiger Name: spastic paraplegia 20 (Troyer syndrome)
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 75-84 kDa
• GenBank-Zugangsnummer: BC047083
• Gene symbol: Spartin
• Gene ID (NCBI): 23111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.