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Spermidine synthase Monoklonaler Antikörper

Spermidine synthase Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4E10

Kat-Nr. : 68548-1-PBS

Synonyme

PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1

Filter:
  • Western Blot (WB) analysis of various lysates using Spermidine synthase Monoclonal antibody (68548-1-Ig)

    WB analysis using 68548-1-Ig (same clone as 68548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68548-1-Ig (Spermidine synthase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68548-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Spermidine synthase Monoclonal antibody (68548-1-Ig)

    IF Staining of RAW 264.7 using 68548-1-Ig (same clone as 68548-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Spermidine synthase antibody (68548-1-Ig, Clone: 2F4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68548-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68548-1-PBS bindet in WB, IF/ICC, Indirect ELISA Spermidine synthase und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Spermidine synthase fusion protein Ag33919
Vollständiger Name spermidine synthase
Berechnetes Molekulargewicht 302 aa, 34 kDa
Beobachtetes Molekulargewicht34 kDa
GenBank-ZugangsnummerBC000309
Gene symbol Spermidine synthase
Gene ID (NCBI) 6723
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).