Stathmin 1 Polyklonaler Antikörper

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• KD/KO Validated

Stathmin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11157-1-PBS

Synonyme

STMN1, C1orf215, LAP18, Leukemia-associated phosphoprotein p18, OP18

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  WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

  WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells.. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  WB analysis using 11157-1-AP (same clone as 11157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11157-1-AP (same clone as 11157-1-PBS)

  WB result of Stathmin 1 antibody (11157-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Stathmin 1 transfected HepG2 cells. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Stathmin 1 antibody (11157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11157-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Stathmin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Stathmin 1 fusion protein Ag1633
• Vollständiger Name: stathmin 1/oncoprotein 18
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC014353
• Gene symbol: Stathmin 1
• Gene ID (NCBI): 3925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.