Supervillin Polyklonaler Antikörper

Supervillin Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27524-1-PBS

Synonyme

SVIL

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  WB analysis using 27524-1-AP (same clone as 27524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27524-1-AP (Supervillin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27524-1-AP (same clone as 27524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 27524-1-AP (same clone as 27524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 27524-1-AP (same clone as 27524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27524-1-PBS bindet in WB, IHC, Indirect ELISA Supervillin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Supervillin fusion protein Ag26196
• Vollständiger Name: supervillin
• Berechnetes Molekulargewicht: 248 kDa
• Beobachtetes Molekulargewicht: 205 kDa
• GenBank-Zugangsnummer: NM_003174
• Gene symbol: SVIL
• Gene ID (NCBI): 6840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Supervillin (SVIL) belongs to the villin/gelsolin superfamily of actin-binding proteins involved in many cellular processes. A non-muscle-specific form of Supervillin activates androgen receptor activity. Supervillin another isoform, called Archvillin, is expressed in skeletal and cardiac muscle tissue where it plays a role in both muscle fiber structure and signaling. Mutations in Supervillin are associated with myofibrillar Myopathy10 (MFM10), which causes myopathy with myofibrillar disorganization and autophagic vacuoles(PMID: 9867483, 32779703).