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Surfactant protein D Polyklonaler Antikörper

Surfactant protein D Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11839-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Surfactant protein D Polyclonal antibody (11839-1-AP)

    WB analysis using 11839-1-AP (same clone as 11839-1-PBS)

    Mouse lung tissue was subjected to SDS PAGE followed by western blot with 11839-1-AP (Surfactant protein D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using Surfactant protein D Polyclonal antibody (11839-1-AP)

    IP experiment of mouse lung using 11839-1-AP (same clone as 11839-1-PBS)

    IP result of anti-Surfactant protein D (IP:11839-1-AP, 3ug; Detection:11839-1-AP 1:300) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant protein D Polyclonal antibody (11839-1-AP)

    IHC staining of mouse lung using 11839-1-AP (same clone as 11839-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11839-1-AP (Surfactant protein D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant protein D Polyclonal antibody (11839-1-AP)

    IHC staining of mouse lung using 11839-1-AP (same clone as 11839-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11839-1-AP (Surfactant protein D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11839-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11839-1-PBS bindet in WB, IHC, IP, Indirect ELISA Surfactant protein D und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Surfactant protein D fusion protein Ag2461
Vollständiger Name surfactant protein D
Berechnetes Molekulargewicht 375 aa, 38 kDa
Beobachtetes Molekulargewicht 37-40 kDa
GenBank-ZugangsnummerBC022318
Gene symbol Surfactant protein D
Gene ID (NCBI) 6441
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Surfactant protein-D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.