Surfactant protein D Rekombinanter Antikörper

Surfactant protein D Rekombinant Antikörper für WB, IHC, IP, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IP, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250571G8

Kat-Nr. : 85985-1-PBS

Synonyme

Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd

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  WB analysis using 85985-1-RR (same clone as 85985-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85985-1-RR (Sftpd antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02322-1

  Sandwich ELISA standard curve of MP02322-1, Mouse Surfactant protein D Recombinant Matched Antibody Pair - PBS only. 85985-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2924. 85985-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP02322-1

  The mean Surfactant protein D concentration was determined to be 47.1 ng/mL in mouse lung tissue extract based on a 5.3 mg/mL extract load.

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  Sample test of MP02322-1

  Mouse lung, cut into 1-2 mm pieces, was cultured unstimulated or stimulated with 1 μg/mL LPS for 3 days. The mean Surfactant protein D concentration was undetectable in unstimulated supernatant, 7.4 ng/mL in LPS stimulated supernatant.

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  Sample test of MP02322-1

  Serum of sixteen mice was measured. The Surfactant protein D concentration of detected samples was determined to be 293.4 pg/mL with a range of 164.4-696.5 pg/mL.

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  IP experiment of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

  IP result of anti-Surfactant protein D (IP:85985-1-RR, 4ug; Detection:85985-1-RR 1:3000) with mouse lung tissue lysate 1760 ug. This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lung using 85985-1-RR (same clone as 85985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 85985-1-RR (same clone as 85985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 85985-1-RR (same clone as 85985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85985-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85985-1-PBS bindet in WB, IHC, IP, Sandwich ELISA, Indirect ELISA, Sample test Surfactant protein D und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Surfactant protein D fusion protein Eg2924
• Vollständiger Name: surfactant associated protein D
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: NM_009160.2
• Gene symbol: Sftpd
• Gene ID (NCBI): 20390
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Surfactant protein D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.