Surfactant protein D Rekombinanter Antikörper

Surfactant protein D Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250571G8

Kat-Nr. : 85985-1-RR

Synonyme

Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd

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  WB analysis using 85985-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85985-1-RR (Sftpd antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 85985-1-RR

  IP result of anti-Surfactant protein D (IP:85985-1-RR, 4ug; Detection:85985-1-RR 1:3000) with mouse lung tissue lysate 1760 ug.

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  IHC staining of rat lung using 85985-1-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 85985-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 85985-1-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 85985-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85985-1-RR (Surfactant protein D antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Rattenlungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85985-1-RR bindet in WB, IHC, IP, ELISA Surfactant protein D und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Surfactant protein D fusion protein Eg2924
• Vollständiger Name: surfactant associated protein D
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: NM_009160.2
• Gene symbol: Sftpd
• Gene ID (NCBI): 20390
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Surfactant protein D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.