Synaptophysin Monoklonaler Antikörper

Synaptophysin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4E12C4

Kat-Nr. : 67864-1-PBS

Synonyme

SYP, 4E12C4, MRX96, SYN

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  WB analysis using 67864-1-Ig (same clone as 67864-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  WB analysis using 67864-1-Ig (same clone as 67864-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IHC staining of rat pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 67864-1-Ig (same clone as 67864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67864-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA Synaptophysin und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Synaptophysin fusion protein Ag11803
• Vollständiger Name: synaptophysin
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC064550
• Gene symbol: Synaptophysin
• Gene ID (NCBI): 6855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)