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Synaptophysin Monoklonaler Antikörper

Synaptophysin Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E12C4

Kat-Nr. : CL488-67864

Synonyme

synaptophysin, SYP

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Monocl (CL488-67864)

    IF Staining of mouse brain using CL488-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Monocl (CL488-67864)

    IF Staining of mouse brain using CL488-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated Synaptophysin Monocl (CL488-67864)

    FC experiment of PC-12 using CL488-67864

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Synaptophysin (CL488-67864, Clone:4E12C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushirngewebe
Erfolgreiche Detektion in FCPC-12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67864 bindet in IF, FC (Intra) Synaptophysin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Synaptophysin fusion protein Ag11803
Vollständiger Name synaptophysin
Berechnetes Molekulargewicht 313 aa, 34 kDa
GenBank-ZugangsnummerBC064550
Gene symbol SYP
Gene ID (NCBI) 6855
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Synaptophysin antibody CL488-67864Protokoll herunterladen
FC protocol for CL Plus 488 Synaptophysin antibody CL488-67864Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen