Synaptophysin Monoklonaler Antikörper

Synaptophysin Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4E12C4

Kat-Nr. : CL594-67864

Synonyme

SYP, SYN, MRX96, 4E12C4

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  IF Staining of mouse brain using CL594-67864

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-67864

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-67864

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:2000.

• 

  IF Staining of mouse brain using CL594-67864

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:2000.

• 

  FC experiment of PC-12 using CL594-67864

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Synaptophysin (CL594-67864, Clone:4E12C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): PC-12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67864 bindet in IF-P, FC (Intra) Synaptophysin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Synaptophysin fusion protein Ag11803
• Vollständiger Name: synaptophysin
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• GenBank-Zugangsnummer: BC064550
• Gene symbol: Synaptophysin
• Gene ID (NCBI): 6855
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)