Synaptotagmin-1 Monoklonaler Antikörper

Synaptotagmin-1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H1H2

Kat-Nr. : 68043-1-PBS

Synonyme

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

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  WB analysis using 68043-1-Ig (same clone as 68043-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68043-1-PBS bindet in WB, IHC, Indirect ELISA Synaptotagmin-1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Synaptotagmin-1 fusion protein Ag6071
• Vollständiger Name: synaptotagmin I
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC058917
• Gene symbol: Synaptotagmin-1
• Gene ID (NCBI): 6857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).