CD138/Syndecan-1 Rekombinanter Antikörper

CD138/Syndecan-1 Rekombinant Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241278E8

Kat-Nr. : 84069-8-RR

Synonyme

CD138, Sdc1, SYND1, Synd-1, Syndecan-1

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  IHC staining of mouse spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84069-8-RR

  Biolayer interferometry (BLl) kinetic assays of 84069-8-RR against Mouse CD138/Syndecan-1 were performed. The affinity constant is 1.15 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Tonsillitisgewebe, Maus-Kolongewebe, Maushautgewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84069-8-RR bindet in IHC, ELISA CD138/Syndecan-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD138/Syndecan-1 fusion protein Eg1221
• Vollständiger Name: syndecan 1
• Berechnetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_011519.2
• Gene symbol: Sdc1
• Gene ID (NCBI): 20969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.