TAC1/Substance P Polyklonaler Antikörper

TAC1/Substance P Polyklonal Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28599-1-AP

Synonyme

TAC1, C-terminal-flanking peptide, Protachykinin 1, Protachykinin-1, TAC 1

---

• 

  IHC staining of mouse brain using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hypothalamus using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens)..

• 

  IHC staining of human hypothalamus using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IF Staining of mouse brain using 28599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 28599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 28599-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hypothalamus-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28599-1-AP bindet in IHC, IF/ICC, IF-P, ELISA TAC1/Substance P und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAC1/Substance P fusion protein Ag23477
• Vollständiger Name: tachykinin, precursor 1
• Berechnetes Molekulargewicht: 129 aa, 15 kDa
• GenBank-Zugangsnummer: BC018047
• Gene symbol: TAC1
• Gene ID (NCBI): 6863
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAC1, also known as Protachykinin-1, is a gene that encodes for a family of neuropeptides known as tachykinins. These peptides are characterized by their ability to rapidly stimulate contraction of intestinal muscle, hence the name "tachykinins". The TAC1 gene and its products are widely distributed in the nervous system, with α-TAC1 mRNA localized in numerous neurons of the brain. β-TAC1 and γ-TAC1 are predominantly expressed in intrinsic enteric neurons and sensory neurons. Additionally, the expression of TAC1, SP, and NKA is observed in various peripheral neurons and non-neural tissues such as the salivary gland, heart, skin, spleen, adrenal gland, and more.