TACC2 Polyklonaler Antikörper

TACC2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11407-1-AP

Synonyme

Anti Zuai 1, AZU 1, ECTACC, TACC2

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 11407-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11407-1-AP (TACC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11407-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11407-1-AP (TACC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11407-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11407-1-AP (TACC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11407-1-AP

  Immunofluorescent analysis of A431 cells using 11407-1-AP (TACC2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11407-1-AP bindet in WB, IHC, IF,ELISA TACC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TACC2 fusion protein Ag1923
• Vollständiger Name: transforming, acidic coiled-coil containing protein 2
• Berechnetes Molekulargewicht: 2948 aa, 309 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC015736
• Gene symbol: TACC2
• Gene ID (NCBI): 10579
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transforming acidic coiled-coil (TACC) proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. TACC2, also known as anti-zuai-1 (AZU-1), was abundantly expressed in non- and premalignant cells and tissues but was appreciably reduced in breast tumor cell types and in primary tumors, thus TACC2 may act as a tumor suppressor protein and represent a tumor progression marker. Several transcript variants encoding different isoforms have been found for this gene. Chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubule and the kinetochore. Centrosomal TACC2 plays a role in organizing centrosomal microtubules and is required for mitotic spindle maintenance.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Biomark; High expression of TACC2 in hepatocellular carcinoma is associated with poor prognosis.; Authors - Shakya Manjul
human	WB	F1000Res; Androgen-dependent alternative mRNA isoform expression in prostate cancer cells.; Authors - Jennifer Munkley