TACC3 Polyklonaler Antikörper

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• KD/KO Validated

TACC3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 25697-1-AP

Synonyme

ERIC 1, ERIC1, ERIC-1, Maskin, TACC 3

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  WB analysis using 25697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25697-1-AP (TACC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 25697-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25697-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TACC3 antibody (25697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

25697-1-AP bindet in WB, IHC, IF/ICC, ELISA TACC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TACC3 fusion protein Ag22516
• Vollständiger Name: transforming, acidic coiled-coil containing protein 3
• Berechnetes Molekulargewicht: 838 aa, 91 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC111771
• Gene symbol: TACC3
• Gene ID (NCBI): 10460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors.

Publikationen

Species	Application	Title
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB,IHC	Oncotarget; TACC3 promotes colorectal cancer tumourigenesis and correlates with poor prognosis.; Authors - Yong Du ; KD Validated
human	WB	Cell Death Dis; PMID: 29358577; Authors - Zhi-Rui Lin ; KD Validated
human	WB,IF	Vet Microbiol; Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import; Authors - Lulu Deng ; KD Validated
mouse,human	WB,IHC,IF	Cell Death Dis; Knockdown of TACC3 inhibits tumor cell proliferation and increases chemosensitivity in pancreatic cancer; Authors - Saimeng Shi ; KD Validated
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu