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TACC3 Rekombinanter Antikörper

TACC3 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242527A12

Kat-Nr. : 84940-5-PBS

Synonyme

242527A12, ERIC 1, ERIC1, ERIC-1, Maskin

Filter:
  • Western Blot (WB) analysis of various lysates using TACC3 Recombinant antibody (84940-5-RR)

    WB analysis using 84940-5-RR (same clone as 84940-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84940-5-RR (TACC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TACC3 Recombinant antibody (84940-5-RR)

    IHC staining of human ovary cancer using 84940-5-RR (same clone as 84940-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84940-5-RR (TACC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TACC3 Recombinant antibody (84940-5-RR)

    IF Staining of HeLa using 84940-5-RR (same clone as 84940-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TACC3 antibody (84940-5-RR, Clone: 242527A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84940-5-RR

    Affinity and Kinetic Characterization of 84940-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84940-5-RR against Human TACC3 were performed. The affinity constant is 2.98 nM.

Geprüfte Anwendungen

Produktinformation

84940-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TACC3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TACC3 fusion protein Ag22516
Vollständiger Name transforming, acidic coiled-coil containing protein 3
Berechnetes Molekulargewicht 838 aa, 91 kDa
Beobachtetes Molekulargewicht160 kDa
GenBank-ZugangsnummerBC111771
Gene symbol TACC3
Gene ID (NCBI) 10460
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors.