TACR2 Polyklonaler Antikörper

TACR2 Polyklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 25270-1-AP

Synonyme

Neurokinin A receptor, NK 2 receptor, NK 2R, NK-2 receptor, NK2R

---

• 

  WB analysis of HeLa using 25270-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25270-1-AP (TACR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25270-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25270-1-AP (TACR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 25270-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TACR2 Polyclonal antibody (25270-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

25270-1-AP bindet in WB, IHC, FC (Intra), ELISA TACR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tachykinin receptor 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: NM_001057
• Gene symbol: TACR2
• Gene ID (NCBI): 6865
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The tachykinins represent a family of peptide neurotransmitters including substance P, neurokinin A and neurokinin B, which act through the stimulation of tachykinin NK1, NK2 or NK3 receptors. NK2, also known as TACR2 (tachykinin receptor 2), is a receptor for the tachykinin neurokinin A (neuropeptide substance K). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Publikationen

Species	Application	Title
human	WB	Cancer Cell Int; TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer.; Authors - Wang Jianfeng
rat	IHC	Mol Med Rep; Occurrence of substance P and neurokinin receptors during the early phase of spinal fusion.; Authors - Suiyi Wu