TACSTD2/TROP2 Rekombinanter Antikörper

TACSTD2/TROP2 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250382C9

Kat-Nr. : 86155-1-RR

Synonyme

TACSTD2, Cell surface glycoprotein Trop-2, EGP 1, GA733, GA733 1

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  WB analysis using 86155-1-RR

  HaCaT cells lysate was subjected to SDS PAGE followed by western blot with 86155-1-RR (TACSTD2/TROP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 86155-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86155-1-RR (TACSTD2/TROP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 86155-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86155-1-RR (TACSTD2/TROP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 86155-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86155-1-RR (TACSTD2/TROP2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 86155-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86155-1-RR (TACSTD2/TROP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HaCaT using 86155-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (86155-1-RR, Clone: 250382C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HaCaT-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86155-1-RR bindet in WB, IHC, IF/ICC, ELISA TACSTD2/TROP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TACSTD2/TROP2 fusion protein Eg3329
• Vollständiger Name: tumor-associated calcium signal transducer 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC009409
• Gene symbol: TROP2
• Gene ID (NCBI): 4070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).