TACSTD2/TROP2 Monoklonaler Antikörper

TACSTD2/TROP2 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5A6B1

Kat-Nr. : 68141-1-PBS

Synonyme

TACSTD2, 5A6B1, Cell surface glycoprotein Trop-2, EGP 1, GA733

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  WB analysis of HaCaT using 68141-1-Ig (same clone as 68141-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 68141-1-Ig (TACSTD2/TROP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68141-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68141-1-Ig (same clone as 68141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68141-1-Ig (TACSTD2/TROP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68141-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68141-1-Ig (same clone as 68141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68141-1-Ig (TACSTD2/TROP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68141-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68141-1-Ig (same clone as 68141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68141-1-Ig (TACSTD2 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68141-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 68141-1-Ig (same clone as 68141-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using TACSTD2/TROP2 antibody (68141-1-Ig, Clone: 5A6B1 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68141-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 68141-1-Ig (same clone as 68141-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (68141-1-Ig, Clone: 5A6B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68141-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68141-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA TACSTD2/TROP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TACSTD2/TROP2 fusion protein Ag25775
• Vollständiger Name: tumor-associated calcium signal transducer 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC009409
• Gene symbol: TROP2
• Gene ID (NCBI): 4070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).