TAF1-Specific Polyklonaler Antikörper

TAF1-Specific Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20260-1-PBS

Synonyme

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  WB analysis using 20260-1-AP (same clone as 20260-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20260-1-AP (same clone as 20260-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20260-1-PBS bindet in WB, IHC, Indirect ELISA TAF1-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
• Berechnetes Molekulargewicht: 213 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa, 50 kDa
• GenBank-Zugangsnummer: NM_138923
• Gene symbol: TAF1
• Gene ID (NCBI): 6872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1. It has several isoforms with predicted MW's of 200-250 kDa and 170-180 kDa, it also could get about 50 kDa band which is caused by the lysate preparation.