TAF11 Polyklonaler Antikörper

TAF11 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16114-1-AP

Synonyme

PRO2134, TAF(II)28, TAF11, TAF2I, TAFII 28, TAFII28, TFIID subunit p30 beta

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16114-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16114-1-AP

  IP result of anti-TAF11 (IP:16114-1-AP, 4ug; Detection:16114-1-AP 1:300) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon cancer using 16114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16114-1-AP

  Immunofluorescent analysis of HepG2 cells using 16114-1-AP (TAF11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16114-1-AP bindet in WB, IP, IHC, IF,ELISA TAF11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAF11 fusion protein Ag9039
• Vollständiger Name: TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa
• Berechnetes Molekulargewicht: 211 aa, 23 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC021972
• Gene symbol: TAF11
• Gene ID (NCBI): 6882
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF11, also named as Transcription initiation factor TFIID 28 kDa subunit, is a 211 amino acid protein, which Belongs to the TAF11 family. TAF11 as a core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 . The calcualted molecular weight of TAF11 is 23 kDa, but modified TAF11 is about 28kDa. ( PMID: 10336414)