TAF15 Rekombinanter Antikörper

TAF15 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3L13

Kat-Nr. : 82860-1-PBS

Synonyme

RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68, 68 kDa TATA-binding protein-associated factor

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  WB analysis using 82860-1-RR (same clone as 82860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

  WB result of TAF15 antibody (82860-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HeLa cells. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TAF15 antibody (82860-1-RR, Clone: 3L13 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).CoraLite® Plus 488-Phalloidin (green) This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82860-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82860-1-RR against Human TAF15 were performed. The affinity constant is 0.974 nM.

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Geprüfte Anwendungen

Produktinformation

82860-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TAF15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TAF15 fusion protein Ag20828
• Vollständiger Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Berechnetes Molekulargewicht: 592 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62-75 kDa
• GenBank-Zugangsnummer: BC046099
• Gene symbol: TAF15
• Gene ID (NCBI): 8148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).