TAF1B Rekombinanter Antikörper

TAF1B Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250162H2

Kat-Nr. : 85885-4-RR

Synonyme

RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63

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  WB analysis of HeLa using 85885-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85885-4-RR (TAF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85885-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85885-4-RR against Human TAF1B were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85885-4-RR bindet in WB, ELISA TAF1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TAF1B fusion protein Ag38211
• Vollständiger Name: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
• Berechnetes Molekulargewicht: 588 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC018137
• Gene symbol: TAF1B
• Gene ID (NCBI): 9014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199).