TAF7 Polyklonaler Antikörper

TAF7 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13506-1-AP

Synonyme

TAF(II)55, TAF2F, TAF7, TAFII 55, TAFII55

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  WB analysis using 13506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13506-1-AP

  IP result of anti-TAF7 (IP:13506-1-AP, 4ug; Detection:13506-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human cervical cancer using 13506-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 13506-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

13506-1-AP bindet in WB, IHC, IP, ChIP, ELISA TAF7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAF7 fusion protein Ag4406
• Vollständiger Name: TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
• Berechnetes Molekulargewicht: 349 aa, 40 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032737
• Gene symbol: TAF7
• Gene ID (NCBI): 6879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF7, also named as Transcription initiation factor TFIID 55 kDa subunit, is a 349 amino acid protein, which belongs to the TAF7 family. TAF7 functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF7 is phosphorylated by CIITA. The calcualted molecular weight of TAF7 is 40 kDa, but phosphorylated TAF7 is about 55kDa. (PMID: 22711989)

Publikationen

Species	Application	Title
mouse	WB	Biomedicines; Busulfan Chemotherapy Downregulates TAF7/TNF-α Signaling in Male Germ Cell Dysfunction; Authors - Daoyuan Huang
human	WB,ChIP	iScience; TAF7 directly targets SAA1 to enhance triple-negative breast cancer metastasis via phosphorylating E-cadherin and N-cadherin; Authors - Wanjun Zhang ; KD Validated