TAF9B Polyklonaler Antikörper

TAF9B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28713-1-PBS

Synonyme

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  WB analysis using 28713-1-AP (same clone as 28713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28713-1-AP (TAF9B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 28713-1-AP (same clone as 28713-1-PBS)

  IP result of anti-TAF9B (IP:28713-1-AP, 4ug; Detection:28713-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28713-1-AP (same clone as 28713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28713-1-AP (TAF9B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28713-1-AP (same clone as 28713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28713-1-AP (TAF9B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28713-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28713-1-PBS bindet in WB, IHC, IP, Indirect ELISA TAF9B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAF9B fusion protein Ag29633
• Vollständiger Name: TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC010350
• Gene symbol: TAF9B
• Gene ID (NCBI): 51616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7, transcription initiation factor TFIID subunit 9-like, transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes, may have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. The calculated MW of TAF9B is 28 kDa, 28713-1-AP can detect a band around 30 kDa.