TAGLN3 Polyklonaler Antikörper

TAGLN3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12246-1-AP

Synonyme

Neuronal protein 22, Neuronal protein NP25, TAGLN3, transgelin 3

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  WB analysis of mouse brain using 12246-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12246-1-AP (TAGLN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12246-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12246-1-AP (TAGLN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12246-1-AP

  IP result of anti-TAGLN3 (IP:12246-1-AP, 4ug; Detection:12246-1-AP 1:2000) with mouse brain tissue lysate 3160 ug.

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  IHC staining of human brain using 12246-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12246-1-AP (TAGLN3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 12246-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12246-1-AP (TAGLN3 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

12246-1-AP bindet in WB, IP, IHC, ELISA TAGLN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAGLN3 fusion protein Ag2891
• Vollständiger Name: transgelin 3
• Berechnetes Molekulargewicht: 22.4 kDa
• Beobachtetes Molekulargewicht: 22.4 kDa
• GenBank-Zugangsnummer: BC015329
• Gene symbol: TAGLN3
• Gene ID (NCBI): 29114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 3 protein. It may cross-react with other two transgenlins based on the sequence similarity.

Publikationen

Species	Application	Title
mouse	IHC	Sci Rep; microRNA regulatory circuits in a mouse model of inherited retinal degeneration.; Authors - Arpad Palfi