TAOK2 Polyklonaler Antikörper

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• KD/KO Validated

TAOK2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21188-1-AP

Synonyme

hKFC C, KIAA0881, Kinase from chicken homolog C, MAP3K17, PSK, PSK 1, PSK1, TAO kinase 2, TAO1, TAO2, TAOK2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 21188-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21188-1-AP

  WB result of TAOK2 antibody (21188-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-control and sh-TAOK2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 21188-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21188-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21188-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21188-1-AP

  IP Result of anti-TAOK2 (IP:21188-1-AP, 4ug; Detection:21188-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human liver cancer using 21188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21188-1-AP (TAOK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21188-1-AP (TAOK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, L02-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 3 publications below

Produktinformation

21188-1-AP bindet in WB, IP, IHC, IF, ELISA TAOK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAOK2 fusion protein Ag15521
• Vollständiger Name: TAO kinase 2
• Berechnetes Molekulargewicht: 1235 aa, 138 kDa
• Beobachtetes Molekulargewicht: 138-150 kDa
• GenBank-Zugangsnummer: BC142663
• Gene symbol: TAOK2
• Gene ID (NCBI): 9344
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAOK2(Serine/threonine-protein kinase TAO2) is also named as KIAA0881, MAP3K17, PSK, PSK1 and belongs to the protein kinase superfamily. TAOK2 is known to modulate gene transcription via its activation of several MAP kinase pathways, including c-Jun amino-terminal kinase (JNK), and has been implicated in the modulation of actin and microtubule dynamics. The time course of TAOK2 expression is consistent with a role for it in neuronal differentiation.(PMID:22735514). The immunogen of this antibody is 742-969 aa of human TAOK2 protein isoform Alpha.This antibody mainly detects the isoform 1/3/4. It can't detect the isoform 2.

Publikationen

Species	Application	Title
human	WB,IF	Am J Hum Genet; Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.; Authors - David T Burns
human	WB,IF	Acta Neuropathol Commun; A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.; Authors - Caterina Giacomini
human	WB,IF	Mol Cancer Ther; Targeting TAO kinases using a new inhibitor compound delays mitosis and induces mitotic cell death in centrosome amplified breast cancer cells.; Authors - Chuay-Yeng Koo
Monkey	WB	J Biol Chem; Prostate-derived sterile 20-like kinases (PSKs/TAOKs) phosphorylate tau protein and are activated in tangle-bearing neurons in Alzheimer disease.; Authors - Tavares Ignatius A IA
monkey	WB	J Cell Sci; Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning.; Authors - Ritu Garg