TAOK3 Monoklonaler Antikörper

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TAOK3 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5A9

Kat-Nr. : 67451-1-PBS

Synonyme

JIK, 1A5A9, CTCL-associated antigen HD-CL-09, Cutaneous T-cell lymphoma-associated antigen HD-CL-09, Dendritic cell-derived protein kinase

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  WB analysis using 67451-1-Ig (same clone as 67451-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  WB analysis using 67451-1-Ig (same clone as 67451-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67451-1-Ig (same clone as 67451-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67451-1-Ig (same clone as 67451-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67451-1-Ig (same clone as 67451-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67451-1-Ig (same clone as 67451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 67451-1-Ig (same clone as 67451-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67451-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67451-1-PBS bindet in WB, IHC, IF-P, ELISA TAOK3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TAOK3 fusion protein Ag29146
• Vollständiger Name: TAO kinase 3
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: BC002756
• Gene symbol: TAOK3
• Gene ID (NCBI): 51347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).