TAP1 Polyklonaler Antikörper

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• KD/KO Validated

TAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11114-1-PBS

Synonyme

RING4, PSF-1, PSF1, EC:7.4.2.14, ATP-binding cassette sub-family B member 2

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  WB analysis using 11114-1-AP (same clone as 11114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11114-1-AP (same clone as 11114-1-PBS)

  WB result of TAP1 antibody (11114-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAP1 transfected HeLa cells. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  WB analysis using 11114-1-AP (same clone as 11114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 11114-1-AP (same clone as 11114-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 11114-1-AP (same clone as 11114-1-PBS)

  IP result of anti-TAP1 (IP:11114-1-AP, 4ug; Detection:11114-1-AP 1:3000) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11114-1-AP (same clone as 11114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11114-1-AP (same clone as 11114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11114-1-AP (same clone as 11114-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using TAP1 antibody 11114-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11114-1-AP (same clone as 11114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11114-1-AP (TAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11114-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11114-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAP1 fusion protein Ag1619
• Vollständiger Name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 70-81 kDa
• GenBank-Zugangsnummer: BC014081
• Gene symbol: TAP1
• Gene ID (NCBI): 6890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.